Noor H. Mohammad1* Abdul Kareem A. AL‐Kazaz1 Ali H. Ad'hiah2
1-Department of Biotechnology, College of Science, University of Baghdad, Iraq.
2- Tropical-Biological Researches Unit, College of Science, University of Baghdad
In this case‐control design study , 50 clinically diagnosed Polycystic Ovarian Syndrome (PCOS) in Iraqi female and 20 healthy Iraqi female were enrolled for analysis of the Exon 17 C/T Single Nucleotide Polymorphisms in INSR gene as predisposing molecular marker for PCOS and its relationship with diabetes. Genomic DNA was extracted from whole blood of each subject using wizard genomic DNA purification kit.Specific primers for Single Nucleotide Polymorphism (SNPs) analysis of INSR Exon 17, Intron 8 and Intron 3 were used Single Nucleotide Polymorphism‐Polymerase Chain Reaction (SNPs‐PCR) amplification reaction. Post PCR Restriction Fragment Length Polymorphism (PCR‐RFLP) was done for PCR‐Products using the Restriction Enzyme PMLI. In conclusion, the Exon 17 C/T Single Nucleotide Polymorphism in INSR gene can consider as predisposing molecular marker for PCOS that can transmitted offspring, CRC and Breast Cancer in Iraqi patients and the Intron 8 Single Nucleotide Polymorphism can consider as predisposing molecular marker for PCOS and Diabetes that can be transmitted offspring in Iraqi patients.