Sample Sidebar Module

This is a sample module published to the sidebar_top position, using the -sidebar module class suffix. There is also a sidebar_bottom position below the menu.

Sample Sidebar Module

This is a sample module published to the sidebar_bottom position, using the -sidebar module class suffix. There is also a sidebar_top position below the search.
قسم التقنيات الاحيائية

Dalal  S.  AL- rubaye*       Heba Mohammed Hamza 1  Thanaa R.  Abdulrahman 2

Email:   dr.tbdalal@gmail.com   *

 1-Department of  Biotechnology,College of Science,Baghdad University,Iraq,Baghdad.

2-Department  of microbiology,College of medicine ,Al-nahrain University.

 

 

Summary

 

A total  of  172 clinical were  obtained over 6 months.  Klebsiella  spp. was detected in 58 (33.7%) samples with a high percentage 29 (50%)  in urine in female  and low percentage 1(1.7%) in pus and burn swabs in male, and the vaginal swab was 1(1.7%). The female to male ratio was 3.1:1.  PCR  detection  showed that  51(87.93%) out of  58  produce 108 bp. product with  rpoB specific primer that represented K. pneumonia. Whereas  7(12.07%)  showed  PCR product  with  343 bp  by  K. oxytoca  specific  primer (peh X), furthermore, the sequences of  two  selected  isolates  showed  that  the  species  related  to K. oxytoca strain CAV1335, and  to K. oxytoca strain CAV1374. Five  selected  isolates  were  re-tested  by  the  gyr A primer,  all were  showed specific  band product  with  441bp.  Sequencing   blast analysis for  these  isolates   showed   that  one  was  related  to   K. pneumoniae  subsp.  pneumoniae  strain  RJF999,  two  isolates  related   to K. pneumoniae strain 17265 GyrA,  one related to  K. oxytoca  strain 7102 GyrA  and one  related to K. pneumonia isolate  103  GyrA  gene.  Phylogenetic tree analysis  showed the relation of 3 K. pneumoniae isolates to USA and UK strains  and  one with the Asian strains,  and 2  K. oxytoca  isolates  have  a relation within the Iranian strains and one has a genetic variation.                                                       

                                                                                                                               
    Key words: K.oxytoca, PCR, sequencing, phylogeny.