Sample Sidebar Module

This is a sample module published to the sidebar_top position, using the -sidebar module class suffix. There is also a sidebar_bottom position below the menu.

Sample Sidebar Module

This is a sample module published to the sidebar_bottom position, using the -sidebar module class suffix. There is also a sidebar_top position below the search.
قسم علوم الحياة

Raghda Sadiq Salih* , Ban Noori Al- Qadhi

Department of Biology, College of Science, University of Baghdad, Iraq

*Email: raghdaS1990@yahoo.com

 

Abstract

Leishmania parasite is the causative agent of different clinical diseases. Fetal calf serum (FCS) is the most expensive and main part ingredient of the Leishmania cultures. In this study  the efficacies of different concentrations (10%, 15%,  20%, 25% and 30%) of the filtered  healthy and diabetic human urine were evaluated as a growth stimulator of Leishmania in culture media and as alternative instead of FCS. The results showed that culture media enriched with the filtered healthy human urine in concentration 25% supported the growth significantly (p<0.05) of the parasites number comparison to all studied concentrations and 15% scored significant (p<0.05) the lowest number, and both concentrations showed significant (p<0.05) increase in comparison to control group. While according to gender, healthy males urine at 30% gave significantly (p<0.05)  the highest concentration of parasites number  and 15% of the lowest, whereas healthy females urine at 25% gave the significant (p<0.05) highest concentration of parasites number  in comparison to all studied concentrations and 15% of the lowest, and both concentrations showed significant (p<0.05) increase in comparison to control group. On other hand the results confirmed that culture media without Fetal Calf  Serum (FCS) enriched with filtered healthy human urine in concentration  of 30% gave a significant (p<0.05)  highest concentration of parasites number in comparison to control group and 10% of the lowest. While according to gender, 25% of healthy males urine gave a significant (p<0.05)  highest parasites number in comparison to all studied concentrations and 10% of the lowest, and both concentrations significantly(p<0.05) lower than the control group, whereas healthy females urine at 30% concentration  gave a significant (p<0.05) high parasites number  in comparison to control group and 10% of the lowest in  M199 media without FCS. So healthy females urine at 30% can be use as alternative instead of FCS in M199 media.

On the other hand the current work studied the effect of culture media (M199) enriched with diabetic human urine, the results showed that the higher number of parasites was scored significantly (p<0.05) in concentration of 25% and lower number of parasites was  in concentration of 20%. While according to gender,  diabetic males urine at 25% gave a significant (p<0.05) highest number of parasites  and 10% of the lowest , whereas in diabetic females urine at 30% gave a significant (p<0.05) highest parasites number and 20% of the lowest and both concentrations were significantly (p<0.05) less than the number of parasites in media without urine. In addition the studied of culture media without FCS enriched with diabetic human urine, the results showed a significant (p<0.05) higher number of parasites in concentration of 25% and the lower number of parasites was in concentration of 15% and both concentrations were significantly (p<0.05) less than the number of parasites in media without urine. While according to gender  diabetic males urine at 25% gave significantly (p<0.05)  highest number and 10% of the lowest and both concentrations were significantly (p<0.05) less than the control group, whereas in diabetic females urine at 30% gave a significant (p<0.05) highest number  and 15% of the lowest  and both concentrations were significantly (p<0.05) less than the control group.                            
Finally, female healthy urine scored significantly more parasites number than the male and total urine(healthy, diabetic and control) and may use alternative instead of FCS.

Keywords: Leishmania tropica, FCS, Human urine, Diabetic urine.