Mokhtar J.kadhim *, Bahaa A. Lafta
Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.
Two hundred and ten specimens include urine, blood and ear swab were collected from different hospitals in Baghdad city; 85 (40%) isolates were diagnosed as Proteus spp. with (82%), (11.7%) and (5.8%) in urine, blood and ear swab samples respectively. PCR technique was shown 30(35.3%) isolates positive for Urease C gene that specific for Proteus vulgaris. The ability for chondritinase production was investigated, 24(80%) isolates were able to chondritinase production and the isolate (p17) has higher enzyme activity at (175.2U/ml). The Chondroitinase was purified by short steps included precipitate with 60% ammonium sulfate, dialysis and gel filtration by Sephadex G-150. The enzymatic activity was peaked to (270U/ml) and specific activity (692.3U/mg). The enzymatic activity for purified enzyme was increased significantly (P<0.01) to (563.7U/ml) after treatment with 1M of tyrosine but aspartic acid and glutamic acid were show inhibitory effect on enzyme activity decreased (P<0.01) to (130.U/ml),(147.3U/ml) respectively. The enzymatic activity was increased significantly (P<0.01) to (633.6U/ml) and (610.3U/ml) when treated with 50% of vitamin k and piroxicam respectively. The aim of this study is to investigate effect of amino acids, vitamins and some nonsteroidal anti-inflammatory drugs (NSAIDs) with different concentrations on enzyme activity.
Keywords: Urease C, Proteus vulgaris, Chondroitinase, Tyrosine,Vitamin k, Feldene